Products

DESCRIPTION

Basic Info

Model NO.	GB15938
Host Animal	Mouse
Isotype	Igg
Storage	-20ºC
Package	Bottle with Ice, Individual Foil Pouch
Sample	Available
Transport Package	Standard Export Carton
Specification	100ul/pc
Trademark	Servicebio
Origin	Wuhan, China
HS Code	3502900000
Production Capacity	50000 Milligram/Milligrams Per Month

Product Description

Biological Antibodies Recombinant Mouse mAb Anti-FLAG Tag AntibodyProduct Information

Description	Flag Tag ( DYKDDDDK Tag ) mouse monoclonal antibody
Synonyms	DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
Immunogen	Synthetic peptide : DYKDDDDK
Isotype	IgG
Purity	Affinity purification

Applications

Applications	Dilution	Positive Tissue
WB	1: 2000-1: 5000	Flag-Tag fusion protein

Background

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Note

Storage	Store at -20 ºC for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium-azide, 100 μg/ml BSA and 50% glycerol.

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