Products

DESCRIPTION

Basic Info

Model NO.	polyclonal antibody
Specification	100ug/1mg
Trademark	Bioworld
Origin	China
Production Capacity	3000 Vials

Product Description

Catalog No.	BS7723
Product Name	HSPB8 polyclonal antibody
Applications	WB IHC IF
Alternative Name	Alpha crystallin C chain; Alpha-crystallin C chain; Charcot Marie Tooth disease axonal type 2L; Charcot Marie Tooth disease spinal; CMT2L; CRYAC; DHMN 2; DHMN2; E2 induced gene 1 protein; E2-induced gene 1 protein; E2IG1; H11; Heat shock 22kDa protein 8; Heat shock 27kDa protein 8; Heat shock protein 22; Heat shock protein beta 8; Heat shock protein beta-8; Hereditary motor neuropathy distal; HMN 2; HMN2; HMN2A; HSB8; HSPB 8; Hspb8; HSPB8_HUMAN; OTTHUMP00000239768; Protein kinase H11; Small stress protein like protein HSP22; Small stress protein-like protein HSP22; Spinal muscular atrophy distal adult autosomal dominant;
Swiss-Prot	Q9UJY1
Host	Rabbit
Reactivity	Human,Mouse,Rat
Application_all	WB: 1:500~1:2000 IHC/IF: 1:50~1:200
Product	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity	HSPB8 polyclonal antibody detects endogenous levels of HSPB8 protein.
BiowMW	~ 22 kDa
Note	For research use only, not for use in diagnostic procedure.
Immunogen	Recombinant full length Human HSPB8.

• 
  Western blot analysis of extracts of various cell lines, using HSPB8 antibody at 1:1000 dilution.
• 
  Immunohistochemistry of paraffin-embedded rat lung using HSPB8 antibody at dilution of 1:200 (40x lens).
• 
  Immunohistochemistry of paraffin-embedded human gastric cancer using HSPB8 antibody at dilution of 1:200 (40x lens).
• 
  Immunofluorescence analysis of HeLa cells using HSPB8 antibody. Blue: DAPI for nuclear staining.

Background

Crystallins are the major proteins expressed in the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into a, b and g families; b and g-crystallins compose a superfamily. Crystallins usually contain seven distinctive protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. a-crystallins consist of three gene products, aA, aB and aC-crystallin, which are members of the small heat shock protein family (HSP20). They are induced by heat shock, and act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, a-crystallins do not renature these proteins. Research indicates that binding occurs between membranes and aC-crystallin. The binding site appears to be at the polar-apolar interface in membrane protein (MIP26) and aC-crystallin; the lipid bilayer becomes less mobile with aC-crystallin binding.