Products

DESCRIPTION

Basic Info

Model NO.	BS65510
Certification	CE, ISO9001
Host	Rabbit/Mouse
Reactivity	Human,Mouse,Rat
Transport Package	Refrigerated Transport
Specification	100ug/1mg
Trademark	Bioworld
Origin	China
HS Code	300219000
Production Capacity	3000 Vials

Product Description

Catalog No.	BS65510
Product Name	Integrin alpha 6 monoclonal antibody
Applications	WB,IHC-P
Alternative Name	Integrin alpha-6; Alpha6p; VLA 6 alpha subunit; VLA 6; VLA-6; Integrin α6; CD49 antigen-like family member F; CD49f; Integrin alpha6B; Integrin, alpha 6; ITA6_HUMAN; ITGA6; ITGA6B; Processed integrin alpha-6.
Swiss-Prot	P23229
Host	Rabbit
Reactivity	Human, Mouse
Application_all	WB=1:500-2000 IHC-P=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Product	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Purification&Purity	affinity purified by Protein A
Storage&Stability	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity	Integrin alpha 6 monoclonal Antibody detects endogenous levels of Integrin alpha 6 protein.
BiowMW	~120kDa
Note
Immunogen	Recombinant Human Integrin alpha 6:1-100/1130

• 
  Anti-Integrin alpha 6 at 1/1000 dilution
• 
  Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 6) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Background

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.